Overview

Product name
Mitochondria/Cytosol Fractionation Kit (100 assay)

See all Mitochondria/Cytosol fractionation products (2) ...


Product overview
The Mitochondria/Cytosol Fractionation Kit provides unique formulations of reagents for effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells. The enriched mitochondrial and cytosolic fractions can be used for studying apoptotic and signal transduction pathways to detect translocation of factors interested between the two fractions by Western blotting, ELISA, or other assays. Procedures are simple and easy to perform, no ultracentrifugations and toxic chemicals are involved.


Notes
After opening the kit, store buffers at 4oC. Store Protease Inhibitor Cocktail and DTT at –20oC.


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Store at -20°C, Stable for 6 months at -20°C



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
5X Cytosol Extraction Buffer
            
 
            
1 x 20ml
        
        

            
DTT
            
 
            
1 x 110µl
        
        

            
Mitochondria Extraction Buffer
            
 
            
1 x 10ml
        
        

            
Mitochondria/Cytosol Fractionation Kit
            
ab65320
            
1 x 100 kits
        
        

            
Protease Inhibitor Cocktail
            
 
            
1 vial
        
    


http://www.abcam.com/mitochondria-cytosol-fractionation-kit-100-assay-ab65320.html