土壤微生物基因组中量DNA提取试剂盒-核酸纯化-试剂-生物在线
北京天漠科技开发有限公司
土壤微生物基因组中量DNA提取试剂盒

土壤微生物基因组中量DNA提取试剂盒

商家询价

产品名称： 土壤微生物基因组中量DNA提取试剂盒

英文名称： ZR Soil Microbe DNA MidiPrep™

产品编号： D6101

产品价格： 0

产品产地： 美国

品牌商标： ZYMO RESEARCH

更新时间： null

使用范围： null

北京天漠科技开发有限公司
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  • 地址 : 北京市朝阳区北苑路170号凯旋城E座1809室
  • 邮编 : 100101
  • 所在区域 : 北京
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  • 邮箱 : fjan@zymoresearch.com.cn
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ZR Soil Microbe DNA MidiPrep™

 HIGHLIGHTS

•
Simple, efficient isolation of humic-free, PCR-quality DNA from microbes in soil, sludge, sediment, and sand in as little as 25 minutes including tough-to-lyse bacteria, fungi, algae, and protozoa.
• State-of-the-art, ultra-high density BashingBeads™ are fracture resistant and chemically inert.
• Can be used with any bead mill, disrupter, or vortex that can accommodate standard 50 ml tubes.
• Omits the use of organic denaturants as well as proteinases.


SPECIFICATIONS
• Formats: Bead Beating, Spin/Vacuum Filtration, and Spin Column Purification.
• Sample Sources: DNA is isolated from bacteria, fungi, protozoa, and algae in up to 5 g of soil. However, 2.5 g is recommended for most applications. The amount of sample will vary depending on the composition of the sample: process more material for wet muddy samples and less for dry sandy samples. DNA can also be isolated directly from isolated (pelleted) fungus and bacteria (see protocol).
• DNA Purity: High quality, humic/fulvic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260 nm/280 nm > 1.8, A260 nm/230 nm > 2.0
• DNA Size Limits: Capable of recovering genomic DNA sized fragments from 100 bp to ≥ 40 kb. Typical fragment sizes range from 25 kb-35 kb. If present, parasitic and viral DNA will also be recovered.
• DNA Recovery: Typically, up to ~125 µg total DNA is eluted into ≥150 µl DNA Elution Buffer per sample.
• Equipment: Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter



DESCRIPTION

The ZR Soil Microbe DNA MidiPrep™ is designed for the simple, rapid isolation of humic-free, PCR-quality DNA from microbes in soil. The kit can be used to successfully isolate metagenomic DNA from tough-to-lyse bacteria, fungi, protozoa, and algae that inhabit a range of samples including clay, sandy, silty, peaty, chalky, and loamy soils. Soil samples (≤5 g) are added to a ZR BashingBead™ Lysis/Filtration Tube where microbes are rapidly and efficiently lysed by bead beating in a uniquely designed lysis buffer. Our Fast-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that inhibit PCR. The entire procedure can be performed in as little as 25 minutes, and there is no need for organic denaturants or proteinases. The ZR Soil Microbe DNA MidiPrep™ can also be used to successfully isolate DNA from cultured bacteria, fungi, and yeast. A schematic of the procedure is shown below.
The ZR Soil Microbe DNA MidiPrep™ is designed for the simple, rapid isolation of humic-free, PCR-quality DNA from microbes in soil. The kit can be used to successfully isolate metagenomic DNA from tough-to-lyse bacteria, fungi, protozoa, and algae that inhabit a range of samples including clay, sandy, silty, peaty, chalky, and loamy soils. Soil samples (d
 
The ZR Soil Microbe DNA Kit™ is designed for the simple, rapid isolation of humic-free, PCR-quality DNA from microbes in soil. The kit can be used to successfully isolate DNA from tough-to-lyse bacteria, fungi, protozoa, and algae that inhabit a range of samples including clay, sandy, silty, peaty, chalky, and loamy soils. Soil samples are added to a ZR BashingBead™ Lysis Tube where microbes are rapidly and efficiently lysed by bead beating in a uniquely designed lysis buffer. Our Fast-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that inhibit PCR. The entire procedure can be performed in as little as 15 minutes, and there is no need for organic denaturants or proteinases.
 
The ZR Soil Microbe DNA Kit™ can be used to isolate high quality DNA from a variety of soil types which yields robust products following PCR   The ZR Soil Microbe DNA Kit™ can be used to isolate high quality DNA from a variety of soil types which yields robust products following PCR. Panel A: Physical characteristics of sampled soils (1-5). Panel B: Microbial, genomic DNA was isolated from soil samples (1-5) using the ZR Soil Microbe DNA Kit™. Approximately 10% of the eluted DNA was then separated in a 0.8% (w/v) agarose/ethidium bromide gel. Panels C and D show the results of PCR of microbial DNA isolated from the samples with primers specific for prokaryotic 16S rRNA (C) or eukaryotic rRNA (D). In the figures, the 1 kb size marker (NEB) is as indicated and the arrows show the prokaryotic 16S rRNA and eukaryotic rRNA PCR products.
     
DNA isolated from Saccharomyces cerevisiae (strain TMY18) using the ZR Soil Microbe DNA Kit™ is high-quality and structurally intact. Equivalent amounts of yeast were processed using the ZR Soil Microbe DNA Kit™ or the kits from competitors M and E. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (NEB).
   DNA isolated from Saccharomyces cerevisiae (strain TMY18) using the ZR Soil Microbe DNA Kit™ is high-quality and structurally intact. Equivalent amounts of yeast were processed using the ZR Soil Microbe DNA Kit™ or the kits from competitors M and E. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (NEB).



ORDERING INFORMATION:
Products Description
Protocol
Cat No.
Size

Price

(RMB)

  

ZR Soil Microbe DNA MidiPrep™

 Efficient isolation of humic-free, PCR-quality DNA from microbes in soil.
D6101
25 preps.