• 靶标种属Mouse
• 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG-Fc. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
• 经测试应用ICC, IHC-P, ELISA, WBmore details
• 偶联物HRP

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C.
• 存储溶液Preservative: None
  Constituents: 0.2% BSA, PBS
• 浓度1 mg 浓度为 1 mg/ml 
  
• 纯度Immunogen affinity purified
• 纯化说明Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
• 共轭说明Molar enzyme/ antibody protein ratio is 4:1
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Immunology
   
  • Immunoglobulins
   
  • Heavy Chain
   
  • IgG

  • Secondary antibodies
   
  • anti-Mouse
   
  • IgG
   
  • Enzyme
   
  • HRP

应用

Goat Anti-Mouse IgG Fc (HRP) 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG Fc (HRP) (ab97265)

  IHC image of beta actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97265,  1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is secondary-only at 1/200 dilution.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre