抗赖氨酸乙酰化鼠单克隆泛抗体（克隆号Kac-01)-抗体-抗体-生物在线

抗赖氨酸乙酰化鼠单克隆泛抗体（克隆号Kac-01)

商家询价

产品名称：抗赖氨酸乙酰化鼠单克隆泛抗体（克隆号Kac-01)

英文名称： Pan acetyllysine antibody (clone Kac-01)

产品编号： PTM-101

产品价格： null

产品产地：杭州(Hangzhou China)

品牌商标： PTM

更新时间： null

使用范围： WB IP

杭州景杰生物科技股份有限公司

联系人 :
地址 : 杭州经济技术开发区6号大街452号
邮编 : 310018
所在区域 : 浙江
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邮箱 : market@ptm-biolab.com

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产品详情

Pan anti-acetyllysine mouse mAb(clone Kac-01) Cat#PTM-101
Pack Size:100 μL Price: ￥2400
Immunogen: Acetylated BSA Reactivity: All species expected

Product Description: The mouse monoclonal pan anti-acetyllysine antibody (clone Kac-01) is purified with Protein G affinity chromatography. It specifically recognizes proteins with acetyllysine residues but not the propionyllysine or butyryllysine residues with structural similarity. Though the antibody shares a similar recognition pattern with anti-acetyllysine antibody(Cat#PTM-102, clone Kac-11), it has more selective recognition profile to some acetylated substatrates. In addition the common applications including Western blotting, immunoprecipitation and immunofluorescence, when used in combination with anti-acetyllysine antibody (Cat#PTM-102, clone Kac-11) , this antibody identifies most lysine acetylated peptides in both prokaryotic and eukaryotic cells in proteomics screenings.

Source/Purification: The mouse monoclonal antibody is produced by immunizing animals with acetylated BSA. Antibodies are purified by acetyllysine-conjugated agarose from media of cultured cells.

Specificity: Anti-acetyllysine antibody detects proteins post-translationally modified by acetylation on lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. The antibody has been shown to have more than 500 times selectivity against propionylated BSA, butyrylated BSA and unmodified BSA.

Recommended Applications: ELISA, WB, IF, IP
*WB=Western blotting; IF=Immunofluorescence; IP=Immunopreciptation.
Recommended dilution: WB:1:1000, IF: 1:500; IP: 1:200.
IMPORTANT: For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.
Use at an assay dependent concentration.Optimal dilutions/concentrations should be determined by the end user and maybe assay dependent. Not yet tested in other applications.

Scientific Description: The post-translational ε-amino lysine acetylation of proteins, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of protein acetylation status is impaired in the pathologies of cancer and other diseases. HDACs have become promising targets for anti-cancer drugs.

Storage & Stability: Store product at -20℃. Avoid repeated freeze / thaw cycles. Antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.

**This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Immunogen: Acetylated BSA Reactivity: All species expected Product Description: The mouse monoclonal pan anti-acetyllysine antibody (clone Kac-01) is purified with Protein G affinity chromatography. It specifically recognizes proteins with acetyllysine residues but not the propionyllysine or butyryllysine residues with structural similarity. Though the antibody shares a similar recognition pattern with anti-acetyllysine antibody(Cat#PTM-102, clone Kac-11), it has more selective recognition profile to some acetylated substatrates. In addition the common applications including Western blotting, immunoprecipitation and immunofluorescence, when used in combination with anti-acetyllysine antibody (Cat#PTM-102, clone Kac-11) , this antibody identifies most lysine acetylated peptides in both prokaryotic and eukaryotic cells in proteomics screenings. Source/Purification: The mouse monoclonal antibody is produced by immunizing animals with acetylated BSA. Antibodies are purified by acetyllysine-conjugated agarose from media of cultured cells. Specificity: Anti-acetyllysine antibody detects proteins post-translationally modified by acetylation on lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. The antibody has been shown to have more than 500 times selectivity against propionylated BSA, butyrylated BSA and unmodified BSA. Recommended Applications: ELISA, WB, IF, IP WB=Western blotting; IF=Immunofluorescence; IP=Immunopreciptation. Recommended dilution: WB:1:1000, IF: 1:500; IP: 1:200. IMPORTANT: For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.* Use at an assay dependent concentration.Optimal dilutions/concentrations should be determined by the end user and maybe assay dependent. Not yet tested in other applications.
Scientific Description: The post-translational ε-amino lysine acetylation of proteins, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of protein acetylation status is impaired in the pathologies of cancer and other diseases. HDACs have become promising targets for anti-cancer drugs. Storage & Stability: Store product at -20℃. Avoid repeated freeze / thaw cycles. Antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt. **This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.