SPECIFICATION SHEET

Catalog #:                         L1C00501                   Lot #:        C080301

Description:                    MAb to Neuron Specific Enolase

                                    Monoclonal Antibody to Neuron Specific Enolase (NSE)

Specificity:                       Gamma enolase.  Specific for the gamma subunit without detectable cross-reactivity with the alpha or beta subunits.  Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts. 

Clone:                                 ML01N001

Host Animal:                  Mouse.  Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.          

Isotype:                             IgG_2a

Source:                               Ascites

Immunogen:                Purified neuron-specific enolase from human brain

Format:                              Purified, Liquid

Purification:                  >95% pure (SDS-PAGE).  Protein G chromatography

Concentration:             2 mg/ml (OD280nm, E^0.1% = 1.4)

Affinity Constant:     1x10^-9

Buffer:                               PBS, pH 7.4

Preservative:                  None

Applications:                 Suitable for use in ELISA.  May be used for immunohistochemistry on formalin-fixed and paraffin-embedded sections.  Each laboratory should determine an optimum working titer for use in its particular application.  Other applications have not been tested but use in such assays should not necessarily be excluded.

                                               Recommended pairs in ELISA:

                                                Capture                                 Detection

                                                L1C00501                            L1C00502

Storage:                             Short-term (up to 2 months) store at < –20°C.  Long term (>2 months) store at <–40°C.