免疫原：Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Vimentin aa 400 to the C-terminus (acetyl ). The exact sequence is proprietary.
常规说明
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- 解离常数（KD）KD = 1.10 x 10 -10 MLearn more about KD
- 存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
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- 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR3776
- 同种型IgG
- 研究领域
应用
Our Abpromise guarantee covers the use of ab92547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 |
Abreviews |
说明 |
| ICC/IF |
|
1/250. |
| WB |
|
1/1000 - 1/5000. Predicted molecular weight: 54 kDa. |
| Flow Cyt |
|
1/50.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| IHC-P |
|
1/200 - 1/500. |
靶标
- 功能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
- 组织特异性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
- 疾病相关Cataract 30
- 序列相似性Belongs to the intermediate filament family.
- 结构域The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
- 翻译后修饰Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
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Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker 图像
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![Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)](http://www.abcam.cn/ps/products/92/ab92547/Images/ab92547-254351-ab92547WB3.jpg)
Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) at 1/20000 dilution (purified) + COS-1 cell lysate at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size : 54 kDa
Observed band size : 54 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)](http://www.abcam.cn/ps/products/92/ab92547/Images/ab92547-254350-ab92547WB2.jpg)
Western blot - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
