Introduction

Activated Supports for Immobilization of Antibodies, Antigens, Affinity Ligands and Enzymes

The growth of process-scale affinity chromatography has created the need for a new generation of support matrix materials and coupling chemistries suited for the industrial environment. Classical agarose based supports perform poorly at the large-scale for several reasons. They provide poor flow properties in large columns. The widely used cyanogen bromide coupling chemistry has well-documented problems with bond stability and non-specific adsorption. Additionally, even with more modern chemistries, agarose can shed polysaccharide chains, giving rise to significant ligand leakage under mild operating conditions. Cellufine activated supports provide state-of-the-art laboratory performance at the process-scale without difficulty. The products are based on rigid spherical cellulose beads specially optimized for affinity chromatography to provide very large pore size and high ligand capacity together with high flow rates in large columns. The cellulose backbone offers very low non-specific adsorption without the ligand leakage problems of agarose.

Features •

High flow rates in laboratory and process columns for high throughput • Low ligand leakage due to exceptionally stable coupling chemistry and support matrix • Excellent mechanical, chemical and environmental resistance • High ligand loading capacity • Compatible with high molecular weight ligands and target proteins due to pore size equivalency with 4 % cross-linked agarose media • Unreacted formyl groups easily converted during reduction to neutral hydroxyls for low non-specific adsorption • Built in hydrophilic spacer arms for maximum ligand accessibility and low non-specific adsorption • No media damage or fines generation with extended mixing to allow use of simple coupling apparatus • Ligand coupling occurs under mild conditions in short reaction times • Thermal stability of media allows high temperature reactions • Long shelf-life of unreacted media