基本信息

产品名称Recombinant Human IGF-1 Protein英文名称Recombinant Human IGF-1 Protein规格或纯度Animal Free, >95% SDS-PAGE, Active, E.coli, No tag, 49-188aa生物活性Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <4.6 ng/mL.内毒素水平<0.2 EU/µg表达系统Pichia pastoris种属Human氨基酸49 to 118序列GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA蛋白标签No tagN-末端序列Gly蛋白长度Full length proteinCarrier FreeYesAnimalFreeYesAccession#P05019来源Recombinant预测分子量7.7 KD±0.8kDaSDS-PAGE8.5 kDa, under reducing conditions; 8.5 kDa, under non-reducing conditions.

相关属性

特点与好处·Application:Cell Culture,FuncS,HPLC,MS,SDS-PAGE物理外观Lyophilized储存缓冲液10mM磷酸钠缓冲液复溶Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a储存温度-20°C储存运输条件超低温冰袋运输稳定性与储存Stable at -20℃ to 24 months, stable at 2-8 ℃ to 4 weeks. Prepared as 100μg/ml storage solution, 2~8℃ can be stably stored for a week.

常规信息

细胞定位Secreted.序列相似性Belongs to the insulin family.涉及疾病Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.基因别名IGF, IGF-I, IGFI, MGF英文别名IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Soma别名IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Soma

一般描述

产品描述：

无动物源性：无动物病毒、无致病物质、无外源因子污染，安全性高。

胰岛素样生长因子-1(IGF-1)又称生长因子 C (SomatamedinC)，属于胰岛素 样生长因子家族，在结构上与胰岛素原具有同源性。IGF-1 是一种促有丝分列的多肽生长因子，在体外刺激肌肉、骨、软骨等多种细胞的增殖和存活。IGF-1 能与 IGF-1R、IGF-2R 和胰岛素受体结合，唤醒多种信号通路，参与细胞的增殖、分化、存活、 生长、凋亡和再生。重组人胰岛素样生长因子(IGF-1)由 70 个氨基酸组成，是一种用于细胞培养的生物活性蛋白。

本品系重组酵母菌表达、经多步分离纯化，再经 0.22μm 滤膜过滤除菌后冷冻干燥而制成的冻干粉。

1.避免在高温环境长时间存放；操作过程中应避免污染。

2.仅供研究使用，不得用于动物或人类的诊断或治疗。

生物活性验证方法：利用 IGF-1 对人乳腺癌细胞株 MCF-7 的刺激作用，测定其生物学活性。

产品应用：

广泛用于细胞培养，可增强细胞增殖、细胞生长和细胞分化。

Purity

≥ 95% SDS-PAGE.

Molecular weight informat

M - 2.84 Da (Calculated mass 7711.84 Da)GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA

Additional sequence information

N-terminal glycineFull-length mature chain lacking the signal peptide and both pro-peptides.

Function

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.

Product description:

Animal origin-Free; Animal viruses-Free; Pathogenic substances-Free; Exogenous factor pollution, High safety.

Insulin-like growth factor-1 (IGF-1), also known as growth factor C (SomatamedinC), belongs to the insulin-like growth factor family and has homology with proinsulin in structure. IGF-1 is a peptide growth factor that promotes mitosis, and stimulates the proliferation and survival of various cells such as muscle, bone, and cartilage in vitro. IGF-1 can bind to IGF-1R, IGF-2R and insulin receptors, wake up a variety of signaling pathways, and participate in cell proliferation, differentiation, survival, growth, apoptosis and regeneration. Recombinant human insulin-like growth factor (IGF-1) consists of 70 amino acids and is a bioactive protein used in cell culture.

This lyophilized powder is made of recombinant yeast expression, multi-step separation and purification, and then 0.22μm membrane filter sterilization and freeze-drying.

Product application：

Widely used in cell culture to enhance cell proliferation, cell growth and cell differentiation.

图片

Protein Bioactivity: Recombinant Human IGF-1 Protein (rp147252)

Recombinant Human IGF-1 Protein (rp147252)-Protein Bioactivity

Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <4.6ng/mL.

SDS-PAGE: Recombinant Human IGF-1 Protein (rp147252)

Recombinant Human IGF-1 Protein (rp147252)-SDS-PAGE

5μg/lane of Recombinant Human IGF-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 8.5 kDa.