• 经测试应用WB, IHC-P, ICC/IFmore details
• 种属反应性
  与反应: Human
  预测可用于: Mouse, Rat, Cow
• 免疫原

  Synthetic peptide corresponding to a region within internal sequence amino acids 89-183 of Human 14-3-3 alpha + beta (UniProt ID: P31946).

• 阳性对照
  • 293T, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysate; HeLa cells; Human Ca922 xenograft tissue

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• 存储溶液pH: 7.00
  Preservative: 0.01% Thimerosal (merthiolate)
  Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
• 浓度100 µl 浓度为 9.06 mg/ml 
  
• 纯度Protein A purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neural Signal Transduction

  • Signal Transduction
   
  • Adapters
   
  • Cytoplasmic

  • Stem Cells
   
  • Signaling Pathways
   
  • Wnt
   
  • 14-3-3

  • Cell Biology
   
  • Cell Cycle
   
  • Cell Division
   
  • Other cell division antibodies

应用

靶标

• 功能Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.
• 序列相似性Belongs to the 14-3-3 family.
• 翻译后修饰The alpha, brain-specific form differs from the beta form in being phosphorylated.
  Isoform Short contains a N-acetylmethionine at position 1.
• 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Information by UniProt
• 数据库链接
  • Entrez Gene: 286863 Cow
  • Entrez Gene: 7529 Human
  • Entrez Gene: 54401 Mouse
  • Entrez Gene: 56011 Rat
  • Omim: 601289 Human
  • SwissProt: P68250 Cow
  • SwissProt: P31946 Human
  • SwissProt: Q9CQV8 Mouse

  see all
• 别名
  • 14 3 3 alpha antibody
  • 14 3 3 protein beta/alpha antibody
  • 14-3-3 protein beta/alpha antibody

  see all

Anti-14-3-3 alpha + beta antibody 图像

• 

  Western blot - Anti-14-3-3 alpha + beta antibody (ab155032)
  Anti-14-3-3 alpha + beta antibody (ab155032) at 1/1000 dilution + Raji whole cell lysate at 30 µg
  
  Predicted band size : 28 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 alpha + beta antibody (ab155032)
  Immunofluorescence analysis of methanol fixed HeLa cells, labeling 14-3-3 alpha + beta using ab155032 at 1/200 dilution. Lower image costained with Hoechst 33342.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 alpha + beta antibody (ab155032)
  Immunohistochemical analysis of paraffin embedded Human Ca922 xenograft tissue, labeling 14-3-3 alpha + beta using ab155032 at 1/500 dilution.