abcam小鼠单克隆抗体Anti-Vimentin抗体[RV202]-抗体-抗体-生物在线
深圳市宇德立生物科技有限公司
abcam小鼠单克隆抗体Anti-Vimentin抗体[RV202]

abcam小鼠单克隆抗体Anti-Vimentin抗体[RV202]

商家询价

产品名称： abcam小鼠单克隆抗体Anti-Vimentin抗体[RV202]

英文名称： CTRCT30 antibody Epididymis luminal protein 113 antibody FLJ36605 antibody HEL113 antibody VIM antibody VIME_HUMAN antibody Vimentin antibody

产品编号： ab8978

产品价格： null

产品产地： 英国

品牌商标： abcam

更新时间： null

使用范围：

深圳市宇德立生物科技有限公司
  • 联系人 :
  • 地址 : 深圳市宝安区西乡宝民二路贤基大厦4E
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  • 所在区域 : 广东
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应用

Our Abpromise guarantee covers the use of ab8978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Abreviews 说明
Flow Cyt   1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC   1/20.
IHC-Fr   Use at an assay dependent concentration. Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection. For PFA fixed tissue use at 1/1000.
WB   1/100 - 1/1000. Detects a band of approximately 57 kDa.
IHC-FoFr   1/500.
IP   Use at an assay dependent concentration.
IHC-P   Use at an assay dependent concentration.
ICC/IF   Use at an assay dependent concentration. PubMed: 20097175
IHC (PFA fixed)   1/1000.

靶标

  • 功能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关Cataract 30
  • 序列相似性Belongs to the intermediate filament family.
  • 结构域The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

    Anti-Vimentin antibody [RV202] 图像

    • Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)
      Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)

      ab8978 vimentin staining of a tonsilar lymphoma. Note that the epithelium (at the left) is negative.

    • TUNEology 波长可调检测卡盒-->