MCF7 (人breast adenocarcinoma cell line)全细胞裂解液- tumor cell line-抗体相关-抗体-生物在线

产品名称： MCF7 (人breast adenocarcinoma cell line)全细胞裂解液- tumor cell line

英文名称： MCF7 (人breast adenocarcinoma cell line)全细胞裂解液- tumor cell line

产品编号： ab29537

产品价格： null

产品产地：英国

品牌商标： abcam

更新时间： null

使用范围：

深圳市宇德立生物科技有限公司

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形式Liquid
存放说明Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
存储溶液Preservative: None
Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
浓度100 µg 浓度为 3.75 mg/ml
裂解物说明Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
研究领域

背景MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.

Our Abpromise guarantee covers the use of ab29537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Abreviews	说明
WB		Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.