多巴胺受体D3抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
多巴胺受体D3抗体

多巴胺受体D3抗体

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产品名称： 多巴胺受体D3抗体

英文名称： Dopamine D3 receptor

产品编号： 1743

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： WB ELISA IHC-P IHC-F IF

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中文名称 多巴胺受体D3抗体
别    名 DRD3; 3dopamine receptor; D; Dopamine receptor D3; DRD3; Dopamine receptor 3; ETM1; FET1; D(3) dopamine receptor; DRD3; DRD3_HUMAN.  

 

研究领域 细胞生物  神经生物学  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:200-800 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 44kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse DRD3:352-446/446 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is expressed in phylogenetically older regions of the brain, suggesting that it plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). Also known as: dopamine D3 receptor; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1.

Subunit:
Interacts with CLIC6. Interacts with GRK4. Interacts with PALM.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.

Tissue Specificity:
Brain.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P30728

Gene ID:
13490

Database links:

Entrez Gene: 1814 Human

Entrez Gene: 13490 Mouse

Entrez Gene: 29238 Rat

Omim: 126451 Human

SwissProt: P35462 Human

SwissProt: P30728 Mouse

SwissProt: P19020 Rat

Unigene: 121478 Human

Unigene: 439735 Mouse

Unigene: 10356 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

多巴胺受体D3常与某些精神、神经疾病有关，如精神分裂症、早发性痴呆等。但最新研究表明多巴胺受体与肿瘤细胞多药耐药有关。
产品图片 Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-Dopamine D3 receptor (bs-1743R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD
Sample:
HepG2 Cell Lysate at 30 ug
U251 Cell Lysate at 30 ug
Primary: Anti-Dopamine D3 receptor (bs-1743R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (bs-1743R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (bs-1743R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.