mRNA-seq简介
mRNA是由DNA的一条链作为模板转录,携带遗传信息,并能指导蛋白质合成的一类单链RNA。随着增强核酸稳定性和递送系统等技术的发展,越来越多的mRNA技术产品从临床试验走向应用,应用范围也不断拓展。 mRNA测序使用oligo d(T)富集方法结合高通量测序研究mRNA的表达和调控,是几乎所有研究的基础。
送样指南
Total RNA:4 μg
细胞:5x106
组织:50 mg
全血、血清、血浆:4 mL
结果展示
经典案例
1. Shu X, Shu S, Cheng H. Genome-Wide mRNA-Seq Profiling Reveals that LEF1 and SMAD3 Regulate Epithelial-Mesenchymal Transition Through the Hippo Signaling Pathway During Palatal Fusion. Genet Test Mol Biomarkers. 2019 Mar;23(3):197-203
2. Han J, Ahmad HI, Jiang X, Liu G. Role of genome-wide mRNA-seq profiling in understanding the long-term sperm maintenance in the storage tubules of laying hens. Trop Anim Health Prod. 2019 Jul;51(6):1441-1447
3. Liu Q, Guo Y, Han R. Effect Mechanism of Electroacupuncture at ST36 on the Injured Extensor Digitorum Longus in the Jumping Rat Model Based on mRNA-Seq Analysis. Biomedicines. 2021 Dec 7;9(12):1849